QUANTIFICATION OF CYANAZINE IN WATER AND SOIL BY A MAGNETIC PARTICLE-BASED ELISA

被引：44

作者：

LAWRUK, TS ^{[1
]}

LACHMAN, CE ^{[1
]}

JOURDAN, SW ^{[1
]}

FLEEKER, JR ^{[1
]}

HERZOG, DP ^{[1
]}

RUBIO, FM ^{[1
]}

机构：

[1] N DAKOTA STATE UNIV,DEPT BIOCHEM,FARGO,ND 58105

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 05期

关键词：

D O I：

10.1021/jf00029a013

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of cyanazine in water and soil was developed using a novel magnetic particle solid phase. Cyanazine was covalently attached to a bovine serum albumin carrier, and the resulting herbicide-protein conjugate was used in rabbits to produce polyclonal antibodies specific for cyanazine. Specificity studies indicate that the antibody can distinguish cyanazine from other structurally similar triazine herbicides, including atrazine. This ELISA has a limit of detection of 0.035 parts per billion (ppb, ng/mL) in water and 3.5 ppb in soil. The standard curve allows quantitation up to 3.0 ppb in water and 300 ppb in soil. The ELISA compares favorably with GC measurements in the analysis of water samples (r = 0.964). Recoveries from fortified soils averaged 86% using a 24-h extraction with methanol/water (75:25 v/v).

引用

页码：747 / 752

页数：6

共 33 条

[1]

[Anonymous], 1989, STANDARD METHODS EXA

[2] CYANAZINE DISSIPATION AS INFLUENCED BY SOIL PROPERTIES [J].