PARTIAL SEQUENCE OF THE PURIFIED PROTEIN CONFIRMS THE IDENTITY OF CDNA CODING FOR HUMAN LYSOSOMAL ALPHA-MANNOSIDASE-B

被引：19

作者：

EMILIANI, C

MARTINO, S

STIRLING, JL

MARAS, B

ORLACCHIO, A

机构：

[1] UNIV LONDON KINGS COLL, DIV LIFE SCI, LONDON W8 7AH, ENGLAND

[2] UNIV ROMA LA SAPIENZA, DIPARTIMENTO SCI BIOCHIM, ROME, ITALY

[3] UNIV ROMA LA SAPIENZA, CNR, CTR BIOL MOLEC, ROME, ITALY

来源：

BIOCHEMICAL JOURNAL | 1995年 / 305卷

关键词：

D O I：

10.1042/bj3050363

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human lysosomal alpha-mannosidase has been purified by a simple and rapid method in sufficient quantities for the analysis of its subunit composition and partial protein sequencing. Analysis of the N-terminal residues of the 30 kDa polypeptide has enabled a for us to confirm the identity of the recently cloned cDNA that was tentatively identified as that of lysosomal alpha-mannosidase [Nebes and Schmidt (1994) Biochem. Biophys. Res. Commun. 200, 239-245] and to locate the position of this polypeptide within the total deduced amino acid sequence. This finding will therefore provide a firm foundation for the characterization of alpha-mannosidosis mutations.

引用

页码：363 / 366

页数：4

共 16 条

[1] BACHINSKI LL, 1993, AM J HUM GENET, V52, P375
[2] BEAUDET AL, 1989, METABOLIC BASIS INHE, P1603
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] HUMAN MANNOSIDOSIS - ENZYMIC DEFECT
CARROLL, M
ROBINSON, D
WINCHEST.BG
MASSON, PK
DANCE, N
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1972, 49 (02) : 579 - &
[5] PURIFICATION AND COMPARISON OF THE STRUCTURES OF HUMAN-LIVER ACIDIC ALPHA-D-MANNOSIDASE-A AND ALPHA-D-MANNOSIDASE-B
CHENG, SH
MALCOLM, S
PEMBLE, S
WINCHESTER, B
[J]. BIOCHEMICAL JOURNAL, 1986, 233 (01) : 65 - 72
[6] CONTINUOUS GROWTH AND DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC-CELLS IN SUSPENSION CULTURE
COLLINS, SJ
GALLO, RC
GALLAGHER, RE
[J]. NATURE, 1977, 270 (5635) : 347 - 349
[7] TREATMENT OF HL-60 CELLS WITH DIMETHYL-SULFOXIDE INHIBITS THE FORMATION OF BETA-N-ACETYLHEXOSAMINIDASE-S
EMILIANI, C
FALZETTI, F
ORLACCHIO, A
STIRLING, JL
[J]. BIOCHEMICAL JOURNAL, 1990, 272 (01) : 211 - 215
[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[9] PURIFICATION AND CHEMICAL CHARACTERIZATION OF HUMAN ACIDIC ALPHA-D-MANNOSIDASE
LEE, JES
LITTLE, TE
YOSHIDA, A
[J]. ENZYME, 1982, 28 (01) : 33 - 40
[10] The determination of enzyme dissociation constants
Lineweaver, H
Burk, D
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1934, 56 : 658 - 666

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