THE INFLUENCE OF B-CHROMOSOMES ON RDNA ORGANIZATION IN RYE INTERPHASE NUCLEI

被引：28

作者：

DELGADO, M ^{[1
]}

MORAISCECILIO, L ^{[1
]}

NEVES, N ^{[1
]}

JONES, RN ^{[1
]}

VIEGAS, W ^{[1
]}

机构：

[1] UNIV WALES,INST BIOL SCI,ABERYSTWYTH SY23 3DD,DYFED,WALES

来源：

CHROMOSOME RESEARCH | 1995年 / 3卷 / 08期

关键词：

B CHROMOSOMES; INTERPHASE CHROMATIN; RDNA ORGANIZATION; RYE;

D O I：

10.1007/BF00713963

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Patterns of rye rDNA organization in interphase nuclei were studied through the use of in situ hybridization in spreads of root meristem cells from plants with and without B chromosomes (Bs). In cells from plants without Bs each rDNA locus is organized as a single perinucleolar knob of condensed chromatin with decondensed chromatin inside the nucleolus. In plants with Bs there is a marked modification of the pattern, found in more than 23% of nuclei, which involves several regions of condensed chromatin interspersed with decondensed chromatin inside the nucleolus. This B-induced alteration in rDNA interphase organization suggests a change in expression of the rRNA genes located on the A chromosomes probably related to the reduction in nuclear RNA observed previously in plants with Bs. The influence of the Bs on the expression of A chromosome genes, through rearrangement of interphase chromatin, could provide the basis of an explanation for some of the known phenotypic effects of B chromosomes in rye.

引用

页码：487 / 491

页数：5

共 22 条

[1]

BENNETT M D, 1984, P469

[2] IDENTIFICATION OF THE E3900 FAMILY, A 2ND FAMILY OF RYE CHROMOSOME-B SPECIFIC REPEATED SEQUENCES [J].