FURTHER STUDIES ON CHROMATOGRAPHIC SEPARATION OF COLLAGEN MOLECULAR SUBUNITS

A study has been made of three chromatographic methods used for separation of collagen molecular components. Two of the methods use a salt gradient, while the third uses a salt and pH gradient to elute the protein from the column. Optical absorption at 220, 230 and 278 mμ was used for analysis of the column effluent in one of the methods, in order to follow protein concentration and the distribution of the aromatic amino acids. These methods yield minor components which could correspond to acidic and basic telopeptides which are essential to the structure of collagen. © 1968.