MOUSE PHENYLALANINE-HYDROXYLASE - HOMOLOGY AND DIVERGENCE FROM HUMAN PHENYLALANINE-HYDROXYLASE

被引：33

作者：

LEDLEY, FD

GRENETT, HE

DUNBAR, BS

WOO, SLC

机构：

[1] BAYLOR UNIV,HOWARD HUGHES MED INST,HOUSTON,TX 77030

[2] BAYLOR UNIV,INST MOLEC GENET,HOUSTON,TX 77030

[3] BAYLOR UNIV,DEPT PEDIAT,HOUSTON,TX 77030

来源：

BIOCHEMICAL JOURNAL | 1990年 / 267卷 / 02期

关键词：

D O I：

10.1042/bj2670399

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The laboratory mouse represents an important model for the study of phenylalanine metabolism and the pathochemistry of phenylketonuria, yet mouse phenylalanine hydroxylase (PAH) has not been extensively studied. We report the cloning and sequencing of a mouse PAH cDNA, the expression of enzymic activity from the mouse PAH cDNA clone and the identification of mouse PAH and human PAH by two-dimensional PAGE of liver samples. These data confirm the expected homology of mouse PAH and human PAH and suggest differences in the primary sequence and the phosphorylation state of the two enzymes.

引用

页码：399 / 406

页数：8

共 47 条

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