INTERLEUKIN-5 AND INTERLEUKIN-3 INDUCE SERINE AND TYROSINE PHOSPHORYLATIONS OF SEVERAL CELLULAR PROTEINS IN AN INTERLEUKIN-5-DEPENDENT CELL-LINE

被引：63

作者：

MURATA, Y ^{[1
]}

YAMAGUCHI, N ^{[1
]}

HITOSHI, Y ^{[1
]}

TOMINAGA, A ^{[1
]}

TAKATSU, K ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,INST MED IMMUNOL,DEPT BIOL,KUMAMOTO 860,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80899-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and eosinophils. To elucidate the intracellular events via the IL-5 receptor, we have investigated IL-5-induced protein phosphorylation in an IL-5-dependent murine early B-lineage cell line (T88-M). The rapid phosphorylation of a 60-kDa protein at serine residues and of 140-, 92-, 53-, 48-, and 45-kDa proteins at tyrosine residues were induced by the stimulation of T88-M cells with IL-5. T88-M cells were found to proliferate in response to IL-3 as well as IL-5, and the phosphoproteins in T88-M cells could be induced by IL-3 stimulation in a similar manner of those induced by IL-5. Thus, these results suggest that the protein phosphorylation events in T88-M cells may be shared by both pathways via the IL-5 receptor and the IL-3 receptor. © 1990 Academic Press, Inc.

引用

页码：1102 / 1108

页数：7

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