EXPRESSION OF CDNA FOR BATROXOBIN, A THROMBIN-LIKE SNAKE-VENOM ENZYME

被引：23

作者：

MAEDA, M

SATOH, S

SUZUKI, S

NIWA, M

ITOH, N

YAMASHINA, I

机构：

[1] FUJISAWA PHARMACEUT CO LTD,PROD DEV LABS,YODOGAWA KU,OSAKA 532,JAPAN

[2] KYOTO SANGYO UNIV,FAC ENGN,DEPT BIOTECHNOL,KITA KU,KYOTO,KYOTO 603,JAPAN

[3] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT BIOL CHEM,SAKYO KU,KYOTO 606,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1991年 / 109卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123432

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cloned cDNA for batroxobin has been expressed in E. coli. Batroxobin could only be obtained as intracellular aggregates of fusion proteins, fused with a small peptide. To obtain the mature batroxobin, the recognition sequence for thrombin was inserted between the peptide and the mature batroxobin. This fusion protein accumulated in an insoluble form and could easily be purified. After site-specific cleavage of the fusion protein with thrombin, recombinant batroxobin was isolated by preparative electrophoresis. Batroxobin with enzymatic activity was obtained by the refolding of recombinant batroxobin.

引用

页码：632 / 637

页数：6

共 17 条

[1] ELUTION OF PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS, REMOVAL OF SODIUM DODECYL-SULFATE, AND RENATURATION OF ENZYMATIC-ACTIVITY - RESULTS WITH SIGMA SUBUNIT OF ESCHERICHIA-COLI RNA-POLYMERASE, WHEAT-GERM DNA TOPOISOMERASE, AND OTHER ENZYMES [J].