ESTERASES OF THE FLOUNDER (PLATICHTHYS-FLESUS, PLEURONECTIDAE, TELEOSTEI) - DEVELOPMENT OF AN IDENTIFICATION PROTOCOL USING STARCH-GEL ELECTROPHORESIS AND CHARACTERIZATION OF LOCI

被引：5

作者：

BERREBI, P

LANDAUD, P

BORSA, P

RENNO, JF

机构：

[1] Laboratoire de Génétique de l'Institut des Sciences de l'Evolution (URA 327, CNRS), Université Montpellier II, Montpellier Cedex 5, F-34095, Place E. Bataillon

来源：

EXPERIENTIA | 1990年 / 46卷 / 08期

关键词：

enzymatic electrophoresis; enzymatic inhibitors; Esterases; flounder genetics; staining methodology;

D O I：

10.1007/BF01935540

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fish esterases are among the most difficult enzymes to identify using starch gel electrophoresis because of the many loci that are simultaneously active, and especially because of duplication phenomena, satellite bands, and stain trails. In an attempt to simplify and clarify electropherograms, various staining and inhibitory methods were tested on esterases from the flounder Platichthys flesus. A range of migration and staining buffers and substrates were used, as well as chemical inhibition and heat inactivation. A combination of the methods made it possible to distinguish and characterize the five presumed esterase loci. © 1990 Birkhäuser Verlag Basel.

引用

页码：863 / 867

页数：5

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