THE PLASMINOGEN-ACTIVATOR FAMILY FROM THE SALIVARY-GLAND OF THE VAMPIRE BAT DESMODUS-ROTUNDUS - CLONING AND EXPRESSION

被引：95

作者：

KRATZSCHMAR, J

HAENDLER, B

LANGER, G

BOIDOL, W

BRINGMANN, P

ALAGON, A

DONNER, P

SCHLEUNING, WD

机构：

[1] SCHERING AG,INST CELLULAR & MOLEC BIOL,RES LABS,POSTFACH 650311,W-1000 BERLIN 65,GERMANY

[2] UNIV NACL AUTONOMA MEXICO,CTR INVEST INGN GENET & BIOTECNOL,CUERNAVACA 62270,MORELOS,MEXICO

来源：

GENE | 1991年 / 105卷 / 02期

关键词：

RECOMBINANT DNA; CDNA CLONING; NUCLEOTIDE SEQUENCE; FIBRINOLYSIS; SERINE PROTEASE; TRANSIENT EXPRESSION;

D O I：

10.1016/0378-1119(91)90155-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Complementary DNAs coding for four Desmodus rotundus salivary plasminogen activators (DSPAs) were isolated and characterized. The predicted amino acid sequences display structural features also found in tissue-type plasminogen activator. The largest forms (DSPA-alpha-1 and -alpha-2) contain a signal peptide, a finger (F), an epidermal growth factor (EGF), a kringle, and a serine protease domain, whereas DSPA-beta and -gamma lack the F and F-EGF domains, respectively. Additional differences between the four forms suggest that distinct genes code for the members of the DSPA family. Transfection of DSPA-encoding cDNAs, placed under the control of the simian virus 40 late promoter, into COS-1 cells resulted in the secretion of highly fibrin-dependent PAs.

引用

页码：229 / 237

页数：9

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