A PI-FORM OF GLUTATHIONE-S-TRANSFERASE IS A MYELIN-ASSOCIATED AND OLIGODENDROCYTE-ASSOCIATED ENZYME IN MOUSE-BRAIN

被引:118
作者
TANSEY, FA [1 ]
CAMMER, W [1 ]
机构
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT NEUROL,F-140,1300 MORRIS PK AVE,BRONX,NY 10461
关键词
GLUATATHIONE-S-TRANSFERASE; MYELIN; OLIGODENDROCYTES; ASTROCYTES; MOUSE BRAIN; GLIAL CELLS;
D O I
10.1111/j.1471-4159.1991.tb02104.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pi form of glutathione-S-transferase (GST), previously found to be oligodendrocyte associated, has also been found in myelin. In the brains of adult mice, immunocytochemical staining for a pi form of GST was observed in myelinated fibers, as well as oligodendrocytes. In contrast, and as previously found in rats, positive immunostaining for mu forms occurred in astrocytes and not in oligodendrocytes or myelinated fibers. Double immunofluorescence staining strengthened the conclusion that pi was in oligodendrocytes and myelin in mouse brains. The results of enzyme assays performed on brain homogenates and purified myelin indicated that GST specific activities in mouse brain myelin were almost as high (0.8-fold) as those in mouse brain homogenates. Low, but reproducible, GST activities were also observed in myelin purified from rat brains, in which pi had been demonstrated in oligodendrocytes and mu in astrocytes. The pi form was also stained by the immunoblot technique in myelin purified from mouse brain. It was concluded that pi is a myelin associated, as well as oligodendrocyte associated, enzyme in mouse brain, and possibly also in rat brain. This is the first report of localization of GSTs in mouse brain and of GST in myelin.
引用
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页码:95 / 102
页数:8
相关论文
共 32 条
[1]   CHARACTERIZATION AND LOCALIZATION OF GLUTATHIONE-S-TRANSFERASES IN RAT-BRAIN AND BINDING OF HORMONES, NEUROTRANSMITTERS, AND DRUGS [J].
ABRAMOVITZ, M ;
HOMMA, H ;
ISHIGAKI, S ;
TANSEY, F ;
CAMMER, W ;
LISTOWSKY, I .
JOURNAL OF NEUROCHEMISTRY, 1988, 50 (01) :50-57
[2]  
ABRAMOVITZ M, 1987, J BIOL CHEM, V262, P7770
[3]   GLYCEROL PHOSPHATE DEHYDROGENASE, GLUCOSE-6-PHOSPHATE-DEHYDROGENASE, AND LACTATE-DEHYDROGENASE - ACTIVITIES IN OLIGODENDROCYTES, NEURONS, ASTROCYTES, AND MYELIN ISOLATED FROM DEVELOPING RAT BRAINS [J].
CAMMER, W ;
SNYDER, DS ;
ZIMMERMAN, TR ;
FAROOQ, M ;
NORTON, WT .
JOURNAL OF NEUROCHEMISTRY, 1982, 38 (02) :360-367
[4]   ANTIBODY AGAINST MOUSE-LIVER 5'-NUCLEOTIDASE IMMUNOSTAINS WHITE MATTER IN THE ADULT-MOUSE CENTRAL-NERVOUS-SYSTEM [J].
CAMMER, W ;
TANSEY, FA ;
SACCHI, R .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1986, 73 (02) :155-167
[5]   DIFFERENTIAL LOCALIZATION OF GLUTATHIONE-S-TRANSFERASE YP-SUBUNIT AND YB-SUBUNIT IN OLIGODENDROCYTES AND ASTROCYTES OF RAT-BRAIN [J].
CAMMER, W ;
TANSEY, F ;
ABRAMOVITZ, M ;
ISHIGAKI, S ;
LISTOWSKY, I .
JOURNAL OF NEUROCHEMISTRY, 1989, 52 (03) :876-883
[6]   CARBONIC-ANHYDRASE IN OLIGODENDROCYTES AND MYELIN IN THE CENTRAL NERVOUS-SYSTEM [J].
CAMMER, W .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1984, 429 (JUN) :494-497
[7]  
CAMMER W, 1983, Developmental Brain Research, V6, P21
[8]  
CORRIGALL AV, 1988, BIOCHEM INT, V16, P443
[9]   GLUTATHIONE-S-TRANSFERASE ACTIVITY IN THE BRAIN - SPECIES, SEX, REGIONAL, AND AGE-DIFFERENCES [J].
DAS, M ;
DIXIT, R ;
SETH, PK ;
MUKHTAR, H .
JOURNAL OF NEUROCHEMISTRY, 1981, 36 (04) :1439-1442
[10]   QUAKING MOUSE - ISOLATION AND CHARACTERIZATION OF MYELIN PROTEIN [J].
GREENFIELD, S ;
NORTON, WT ;
MORELL, P .
JOURNAL OF NEUROCHEMISTRY, 1971, 18 (11) :2119-+