MECHANICAL-PROPERTIES OF ADULT FELINE VENTRICULAR MYOCYTES IN CULTURE

被引:26
作者
POLLACK, PS
CARSON, NL
NUSS, HB
MARINO, TA
HOUSER, SR
机构
[1] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT MED,PHILADELPHIA,PA 19140
[2] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT PHYSIOL,PHILADELPHIA,PA 19140
[3] TEMPLE UNIV,HLTH SCI CTR,SCH MED,DEPT ANAT,PHILADELPHIA,PA 19140
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 260卷 / 01期
关键词
CONTRACTION; TISSUE CULTURE; MYOCYTES; ELECTROPHYSIOLOGY; NOREPINEPHRINE;
D O I
10.1152/ajpheart.1991.260.1.H234
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The contractile and electrophysiological properties of cultured adult feline ventricular myocytes were studied. Cells were field stimulated and contraction was measured using a video-based edge detector. The magnitude of contraction decreased by 36% and the rate of contraction decreased by 52% 2 h after the cells were plated on laminin-coated cover slips. The magnitude and rate of contraction then remained stable for 1 wk. The duration of contraction prolonged and a second component to the twitch frequently, but not invariably, developed after 5 days in culture. This was associated with prolongation of the action potential duration. After 7 days in culture, cells could be divided into two groups based on resting membrane potential. Norepinephrine increased the magnitude of contraction for 5 days after plating. Cultured ventricular myocytes became unresponsive to the effects of norepinephrine after 7 days. Adult cardiac myocytes maintained in primary culture continue to respond to field stimulation and retain many contractile properties for up to 7 days; however, the functional characteristics of these cells do not remain uniform during this time period.
引用
收藏
页码:H234 / H241
页数:8
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