A HIGH-RESOLUTION PHYSICAL MAP OF 2.5-MBP OF THE DOWN-SYNDROME REGION ON CHROMOSOME-21

被引：34

作者：

PATIL, N

PETERSON, A

ROTHMAN, A

DEJONG, PJ

MYERS, RM

COX, DR

机构：

[1] STANFORD UNIV, SCH MED, DEPT GENET, STANFORD, CA 94305 USA

[2] UNIV CALIF SAN FRANCISCO, DEPT PHYSIOL, SAN FRANCISCO, CA 94143 USA

[3] LAWRENCE LIVERMORE NATL LAB, DIV BIOMED SCI, LIVERMORE, CA 94550 USA

来源：

HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/hmg/3.10.1811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The region surrounding D21S55 in band 21q22 of human chromosome 21 has been implicated in the etiology of Down syndrome (DS). In this paper, we report the construction of a high resolution map of a 2.5 Mb region around the marker D21S55. Characterization of YAC clones by accurate size determination, end isolation and marker assignment was used to build a refined YAC-based map. The YAC clones were then used to isolate 284 cosmid clones, covering 2.3 Mb, from a chromosome 21-specific cosmid library. The cosmid clones were ordered into overlapping groups and a restriction map of each group was determined. The groups of cosmids were then ordered and oriented with respect to the YAC-based map. This high resolution map provides the framework for further analysis of the region by transcribed sequence mapping and sequence determination.

引用

页码：1811 / 1817

页数：7

共 21 条

[1] A COSMID CONTIG AND HIGH-RESOLUTION RESTRICTION MAP OF THE 2 MEGABASE REGION CONTAINING THE HUNTINGTONS-DISEASE GENE [J].

BAXENDALE, S ;

MACDONALD, ME ;

MOTT, R ;

FRANCIS, F ;

LIN, C ;

KIRBY, SF ;

JAMES, M ;

ZEHETNER, G ;

HUMMERICH, H ;

VALDES, J ;

COLLINS, FS ;

DEAVEN, LJ ;

GUSELLA, JF ;

LEHRACH, H ;

BATES, GP .

NATURE GENETICS, 1993, 4 (02) :181-&

[2] GENERATION OF A NESTED SERIES OF INTERSTITIAL DELETIONS IN YEAST ARTIFICIAL CHROMOSOMES CARRYING HUMAN DNA [J].

CAMPBELL, C ;

GULATI, R ;

NANDI, AK ;

FLOY, K ;

HIETER, P ;

KUCHERLAPATI, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) :5744-5748

[3] ORDERED SHOTGUN SEQUENCING, A STRATEGY FOR INTEGRATED MAPPING AND SEQUENCING OF YAC CLONES [J].

CHEN, EY ;

SCHLESSINGER, D ;

KERE, J .

GENOMICS, 1993, 17 (03) :651-656

[4] EFFECTIVE AMPLIFICATION OF LONG TARGETS FROM CLONED INSERTS AND HUMAN GENOMIC DNA [J].

CHENG, S ;

FOCKLER, C ;

BARNES, WM ;

HIGUCHI, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5695-5699

[5] CONTINUUM OF OVERLAPPING CLONES SPANNING THE ENTIRE HUMAN CHROMOSOME-21Q [J].

CHUMAKOV, I ;

RIGAULT, P ;

GUILLOU, S ;

OUGEN, P ;

BILLAUT, A ;

GUASCONI, G ;

GERVY, P ;

LEGALL, I ;

SOULARUE, P ;

GRINAS, L ;

BOUGUELERET, L ;

BELLANNECHANTELOT, C ;

LACROIX, B ;

BARILLOT, E ;

GESNOUIN, P ;

POOK, S ;

VAYSSEIX, G ;

FRELAT, G ;

SCHMITZ, A ;

SAMBUCY, JL ;

BOSCH, A ;

ESTIVILL, X ;

WEISSENBACH, J ;

VIGNAL, A ;

RIETHMAN, H ;

COX, D ;

PATTERSON, D ;

GARDINER, K ;

HATTORI, M ;

SAKAKI, Y ;

ICHIKAWA, H ;

OHKI, M ;

LEPASLIER, D ;

HEILIG, R ;

ANTONARAKIS, S ;

COHEN, D .

NATURE, 1992, 359 (6394) :380-387

[6] 3.6-MB GENOMIC AND YAC PHYSICAL MAP OF THE DOWN-SYNDROME CHROMOSOME REGION ON CHROMOSOME-21 [J].

DUFRESNEZACHARIA, MC ;

DAHMANE, N ;

THEOPHILE, D ;

ORTI, R ;

CHETTOUH, Z ;

SINET, PM ;

DELABAR, JM .

GENOMICS, 1994, 19 (03) :462-469

[7]

Epstein C, 1986, CONSEQUENCES CHROMOS

[8] A NOTI RESTRICTION MAP OF THE ENTIRE LONG ARM OF HUMAN CHROMOSOME-21 [J].

ICHIKAWA, H ;

HOSODA, F ;

ARAI, Y ;

SHIMIZU, K ;

OHIRA, M ;

OHKI, M .

NATURE GENETICS, 1993, 4 (04) :361-366

[9] DOWN-SYNDROME PHENOTYPES - THE CONSEQUENCES OF CHROMOSOMAL IMBALANCE [J].

KORENBERG, JR ;

CHEN, XN ;

SCHIPPER, R ;

SUN, Z ;

GONSKY, R ;

GERWEHR, S ;

CARPENTER, N ;

DAUMER, C ;

DIGNAN, P ;

DISTECHE, C ;

GRAHAM, JM ;

HUGDINS, L ;

MCGILLIVRAY, B ;

MIYAZAKI, K ;

OGASAWARA, N ;

PARK, JP ;

PAGON, R ;

PUESCHEL, S ;

SACK, G ;

SAY, B ;

SCHUFFENHAUER, S ;

SOUKUP, S ;

YAMANAKA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (11) :4997-5001

[10]

LEJEUNE J, 1959, CR HEBD ACAD SCI, V248, P1721

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