CHANGES IN PYRIDINE AND ADENINE-NUCLEOTIDE LEVELS IN FRIEND-ERYTHROLEUKEMIA CELLS DURING GROWTH AND DIFFERENTIATION

被引:9
作者
CALDINI, R
CHEVANNE, M
MAGNELLI, L
机构
[1] Istituto di Patologia Generale, Università di Firenze, Firenze, 50134
关键词
D O I
10.1002/jcp.1041510122
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Pyridine and adenine nucleotide levels were measured in Friend erythroleukaemia cells (FELC) stimulated to growth and induced to differentiate by hexamethylene bisacetamide (HMBA) and N'-methylnicotinamide (N'-MNAM). A three-to fourfold increase in the NADP(H) was found to parallel cell growth stimulation in both the presence and absence of differentiation inducers. NAD(H) increased about twofold in control and to a minor extent in HMBA-treated FELC but did not vary significantly in N'-MNAM-treated cells. ATP was significantly higher in control cells stimulated to growth than in resting ones, but it did not vary in inducer-treated cells. These data confirm the relationship between high NADP(H) levels and cell resumption to growth; moreover they show that NAD(H) pool reduction and NAD/NADH ratio rise are associated with the process of FELC differentiation. The activities of NAD pyrophosphorylase and NAD kinase are much more enhanced in growth-stimulated FELC than in resting ones. On the other hand transition from the quiescent to the proliferative state was accompanied by a decrease in the activity of poly(ADP-ribose) polymerase. A decrease in poly(ADP-ribose) polymerase activity was also found in differentiated cells in contrast to controls.
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页码:172 / 179
页数:8
相关论文
共 35 条
[1]   CHARACTERIZATION AND COMPARISON OF POLY(ADENOSINE DIPHOSPHORIBOSE) SYNTHESIS AND DNA-SYNTHESIS IN NUCLEOTIDE-PERMEABLE CELLS [J].
BERGER, NA ;
WEBER, G ;
KAICHI, AS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 519 (01) :87-104
[2]   AMPLIFICATION OF PYRIDINE-NUCLEOTIDE POOLS IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES [J].
BERGER, NA ;
BERGER, SJ ;
SIKORSKI, GW ;
CATINO, DM .
EXPERIMENTAL CELL RESEARCH, 1982, 137 (01) :79-88
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   INTERRELATIONSHIP BETWEEN POLY(ADP-RIB) SYNTHESIS, INTRACELLULAR NAD LEVELS, AND MUSCLE OR CARTILAGE DIFFERENTIATION FROM MESODERMAL CELLS OF EMBRYONIC CHICK LIMB [J].
CAPLAN, AI ;
ROSENBERG, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) :1852-1857
[5]   RELATIONSHIP BETWEEN PYRIDINE-NUCLEOTIDE LEVELS AND RIBONUCLEOTIDE REDUCTASE-ACTIVITY IN YOSHIDA ASCITES HEPATOMA AH-130 [J].
CHEVANNE, M ;
CALDINI, R .
EXPERIMENTAL CELL RESEARCH, 1986, 167 (02) :327-336
[6]  
CHUNG AE, 1971, METHODS ENZYMOLOGY B, V18, P149
[7]  
CLAYCOMB WC, 1976, BIOCHEM J, V154, P387, DOI 10.1042/bj1540387
[8]  
FRIEND C, 1971, P NATL ACAD SCI USA, V68, P378
[9]  
FRIEND C, 1976, HARVEY LECT, V72, P253
[10]   INTRACELLULAR NAD+ CONTENT AND ADP-RIBOSE POLYMERASE-ACTIVITY OF SERUM-STIMULATED BABY HAMSTER-KIDNEY FIBROBLASTS [J].
FURNEAUX, HM ;
PEARSON, CK .
JOURNAL OF CELLULAR PHYSIOLOGY, 1980, 105 (03) :401-407