EFFECT OF THE SELECTIVE LESION OF SEROTONINERGIC NEURONS ON THE REGIONAL DISTRIBUTION OF 5-HT1A RECEPTOR MESSENGER-RNA IN THE RAT-BRAIN

被引:90
作者
MIQUEL, MC [1 ]
DOUCET, E [1 ]
RIAD, M [1 ]
ADRIEN, J [1 ]
VERGE, D [1 ]
HAMON, M [1 ]
机构
[1] UNIV PARIS 06, INST NEUROSCI, DEPT CYTOL, CNRS, UA 1199, F-75230 PARIS 05, FRANCE
来源
MOLECULAR BRAIN RESEARCH | 1992年 / 14卷 / 04期
关键词
SEROTONIN 5-HT1A RECEPTOR MESSENGER RNA; INSITU HYBRIDIZATION; ANTI-5-HT1A RECEPTOR ANTISERUM; IMMUNOAUTORADIOGRAPHY; 5,7-DIHYDROXYTRYPTAMINE; DORSAL RAPHE NUCLEUS;
D O I
10.1016/0169-328X(92)90104-J
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The effects of the selective lesion of serotoninergic neurons by an intra-raphe administration of 5,7-dihydroxytryptamine on the 5-HT1A receptor protein and the 5-HT1A receptor mRNA were examined in various regions of the rat brain using specific antibodies and an anti-sense riboprobe, respectively. Twenty one days after the treatment, the 5-HT1A receptor protein was no longer detected within the dorsal raphe nucleus but was still present in the hippocampus and entorhinal cortex. Quantitative in situ hybridization showed an 85% decrease in the levels of 5-HT1A receptor mRNA within the dorsal raphe nucleus, but no significant change in the hippocampus, interpeduncular nucleus and entorhinal cortex of 5,7-dihydroxytryptamine-treated rats. These data demonstrate that 5-HT1A receptors are synthesized by serotoninergic neurons in the dorsal raphe nucleus, and by neurons located postsynaptically with regard to serotoninergic projections in other areas. The unchanged levels of 5-HT1A receptor mRNA in the hippocampus. interpeduncular nucleus and entorhinal cortex three weeks after the extensive lesion of serotoninergic neurons are consistent with the absence of 5-HT1A receptor up regulation already reported under this condition.
引用
收藏
页码:357 / 362
页数:6
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