PRIMING EFFECT OF PORPHYROMONAS-GINGIVALIS LIPOPOLYSACCHARIDE ON SUPEROXIDE PRODUCTION BY NEUTROPHILS FROM HEALTHY AND RAPIDLY PROGRESSIVE PERIODONTITIS SUBJECTS

被引：43

作者：

SHAPIRA, L ^{[1
]}

GORDON, B ^{[1
]}

WARBINGTON, M ^{[1
]}

VANDYKE, TE ^{[1
]}

机构：

[1] EASTMAN DENT CTR,DEPT PERIODONTOL,ROCHESTER,NY 14620

来源：

JOURNAL OF PERIODONTOLOGY | 1994年 / 65卷 / 02期

关键词：

PORPHYROMONAS GINGIVALIS; PERIODONTITIS; RAPIDLY PROGRESSIVE; NEUTROPHILS; LIPOPOLYSACCHARIDES; SUPEROXIDE;

D O I：

10.1902/jop.1994.65.2.129

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

PRE-INCUBATION OF NEUTROPHILS from rapidly progressive periodontitis (RPP) patients with lipopolysaccharide (LPS) extracted from Polphyromonas gingivalis was found to prime the neutrophils for enhanced FMLP-stimulated superoxide production in a dose-dependent manner. The priming effect of P. gingivalis LPS on neutrophils from control subjects was scanty or without effect at all. Inclusion of human serum in the experimental priming conditions increased the control and RPP neutrophil response by 2 to 3 fold. Blocking of the CD14 receptor on the neutrophil surface with monoclonal antibody eliminated the priming effect. Furthermore, incubation of control neutrophils with P. gingivalis LPS in the presence of serum from RPP patients generated a higher response as compared to incubation with control serum. The data suggest that neutrophil priming described in RPP patients is dependent on a serum factor which alters the neutrophil response to priming agents such as LPS.

引用

页码：129 / 133

页数：5

共 23 条

[1]

ASMAN B, 1984, SCAND J DENT RES, V92, P218

[2] RECOMBINANT HUMAN-TUMOR NECROSIS FACTOR-ALPHA - REGULATION OF N-FORMYLMETHIONYLLEUCYLPHENYLALANINE RECEPTOR AFFINITY AND FUNCTION ON HUMAN-NEUTROPHILS [J].