PARACRINE CA2+ SIGNALING IN-VITRO - SEROTONIN-MEDIATED CELL-CELL COMMUNICATION IN MAST-CELL SMOOTH-MUSCLE COCULTURES

Mast cells are tissue-resident immune cells that are capable of signaling many different cell types in vascularized tissue including epithelia and smooth muscle. We have developed an in vitro coculture system in which secretion of serotonin by a mucosal mast cell line (RBL-2H3) can be studied at a single cell level by measuring Ca2+ transients in fura-2 loaded mast cells and serotonin-sensitive A7r5 smooth muscle cells using fluorescence video microscopy and digital image processing. A7r5 cells elevate intracellular Ca2+ via 5HT(2) receptors in response to bath-applied serotonin with an ED(50) for serotonin of 550nM. Crosslinking IgE receptors with antigen caused Ca2+ transients in the mucosal mast cells. Ca2+ responses in the smooth muscle were detected approximate to 30-240 sec after the initiation of the mast cell Ca2+ responses. Smooth muscle Ca2+ responses were dependent on preloading mast cells with serotonin and were blocked by the 5HT(2) antagonist ketanserin. The timing and magnitude of the smooth muscle responses indicated that secretion from mast cells can lead to focal concentrations of serotonin in the range of 300 nM within 1 min of antigen stimulation. Th is coculture technique has allowed the first direct demonstration of serotonin-mediated signaling between immune cells and vascular elements. (C) 1994 Wiley-Liss, Inc.