DISTRIBUTION OF MICROTUBULES DURING THE GROWTH OF TOBACCO POLLEN TUBES

被引:50
作者
DELCASINO, C [1 ]
LI, YQ [1 ]
MOSCATELLI, A [1 ]
SCALI, M [1 ]
TIEZZI, A [1 ]
CRESTI, M [1 ]
机构
[1] UNIV TUSCIA,DIPARTIMENTO SCI AMBIENTALI,I-01100 VITERBO,ITALY
关键词
NICOTIANA TABACUM; POLLEN TUBE GROWTH; MICROTUBULES; TYROSINATED ALPHA-TUBULIN; ACETYLATED ALPHA-TUBULIN; CONFOCAL LASER SCANNING MICROSCOPE;
D O I
10.1016/0248-4900(93)90248-D
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The distribution of microtubules was investigated in Nicotiana tabacum pollen tubes at different stages of tube growth by immunofluorescence microscopy. Using specific antibodies, the presence of microtubules consisting of different tubulin isoforms was tested. alpha-, beta- and tyrosinated alpha-tubulin were present within the tube, whereas the acetylated form was lacking. The presence of tubulin subunits in pollen tube extracts was also investigated by immunoblotting analyses. The use of a confocal laser scanning microscope integrated with computer-assisted imaging, allowed a detailed visualization of the microtubule distribution and organization. Cytoplasmic microtubules organized as short bundles with various orientations were detected at the apex of long tubes.
引用
收藏
页码:125 / 132
页数:8
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