Mutations at the act1, fadA, fadB, fadC, and fadD loci of Arabidopsis thaliana affect the fatty acid composition of the chloroplast membrane lipids. The mutations were mapped by measuring the fatty acid composition by gas chromatography of more than 3,300 leaf samples from individuals in segregating populations. Linkage analysis of the act1 mutant indicated that it is 35 cM from the an locus on chromosome 1. The fadA locus is on chromosome 4, where it is 18 cM from cer2 and tightly linked to ap2. The fadB locus is on chromosome 3, where it is 28 cM from gl1 and tightly linked to hy2. The fadC locus is 13 cM from ap2 and is tightly linked to cer2 on chromosome 4. The fadD locus is on chromosome 3, where it is 40 cM from gl1 and 3 cM from hy2.