PATCH-CLAMP RECORDINGS FROM THE SOMA AND DENDRITES OF NEURONS IN BRAIN-SLICES USING INFRARED VIDEO MICROSCOPY

被引：666

作者：

STUART, GJ

DODT, HU

SAKMANN, B

机构：

[1] MAX PLANCK INST MED RES,ZELLPHYSIOL ABT,JAHNSTR 29,W-6900 HEIDELBERG 1,GERMANY

[2] MAX PLANCK INST PSYCHIAT,INST KLIN,W-8000 MUNICH 40,GERMANY

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1993年 / 423卷 / 5-6期

关键词：

PATCH CLAMP; BRAIN SLICE; DENDRITE; VIDEO MICROSCOPY; NEOCORTEX;

D O I：

10.1007/BF00374949

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

A description is given of the implementation of infrared differential interference contrast (IR-DIC) video microscopy to an upright compound microscope. Using the improved resolution offered by IR-DIC a procedure is described for making patch-pipette recordings from visually identified neuronal somata and dendrites in brain slices. As an example of the application of this technique to electrophysiological recordings from small neuronal processes in brain slices we describe whole-cell current-clamp and cell-attached and excised patch-clamp recordings from the apical dendrites of layer V pyramidal neurons in slices of rat neocortex.

引用

页码：511 / 518

页数：8

共 13 条

[1] WHOLE CELL RECORDING FROM NEURONS IN SLICES OF REPTILIAN AND MAMMALIAN CEREBRAL-CORTEX [J].