EPIDERMIS AS A SECRETORY TISSUE - AN IN-VITRO TISSUE MODEL TO STUDY KERATINOCYTE SECRETION

被引:43
作者
KATZ, AB [1 ]
TAICHMAN, LB [1 ]
机构
[1] SUNY STONY BROOK, SCH DENT MED, DEPT ORAL BIOL & PATHOL, STONY BROOK, NY 11794 USA
关键词
CULTURE; EPITHELIAL; SECRETED PROTEIN;
D O I
10.1111/1523-1747.ep12371732
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In addition to protective functions, keratinocytes secrete a variety of effector molecules that may have local or distant effects. To explore the secretory activity of keratinocytes we have developed a two-chamber culture model in which a fully differentiated stratified epithelium is present in the upper chamber and secreted protein is collected in a lower chamber. A collagen matrix is not used and during collection of secreted protein, 3T3 feeder cells are absent. Keratinocytes secrete protein at the rate of 0.67 mu g/h/10(6) cells, encompassing a molecular weight range from several kD to over 180 kD. Secretion is an unexplored area of keratinocyte biology and this model will allow investigation of this activity.
引用
收藏
页码:55 / 60
页数:6
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