STUDIES OF THE NEUTRAL TRISACCHARIDES OF GOAT (CAPRA-HIRCUS) COLOSTRUM AND OF THE ONE-DIMENSIONAL AND 2-DIMENSIONAL H-1 AND C-13 NMR-SPECTRA OF 6'-N-ACETYLGLUCOSAMINYLLACTOSE

被引：68

作者：

URASHIMA, T ^{[1
]}

BUBB, WA ^{[1
]}

MESSER, M ^{[1
]}

TSUJI, Y ^{[1
]}

TANEDA, Y ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA

来源：

CARBOHYDRATE RESEARCH | 1994年 / 262卷 / 02期

关键词：

GOAT COLOSTRUM; OLIGOSACCHARIDES; CHEMICAL STRUCTURES; 6'-N-ACETYLGLUCOSEAMINYLLACTOSE; NMR;

D O I：

10.1016/0008-6215(94)84177-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four neutral trisaccharides were isolated from goat colostrum by dialysis, and ion-exchange, activated charcoal column, preparative paper, and Bio-Gel P-4 column chromatography. The following structures were elucidated by GC analysis of hydrolysis products and by 400-MHz H-1 NMR spectroscopy: alpha-L-Fucp-(1-->2)-beta-D-Galp-(1-->4)-D-Glc, alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-D-Glc, beta-D-Galp-(1-->3)-beta-D-Galp-(1-->4)-D-Glc, and beta-D-Galp-(1-->6)-beta-D-Galp-(1-->4)-D-Glc. beta-D-GlcpNAc-(1-->6)-beta-D-Galp-(1-->4)-4-D-Glc, previously reported to be present in goat milk, was not detected in this study. beta-D-GlcpNAc-(1-->6)-beta-D-Galp-(1-->4)-D-Glc (6'-N-acetylglucosaminyllactose) was prepared by beta-D-galactosidase digestion of beta-D-Galp-(1-->3)[beta-D-GlcpNAc-(1-->6)]-beta-D-Galp-(1-->4)-D-Glc (lacto-N-novotetraose) and characterized by one- and two-dimensional NMR spectroscopy at 600 MHz.

引用

页码：173 / 184

页数：12

共 34 条

[1] CORRELATION OF PROTON AND N-15 CHEMICAL-SHIFTS BY MULTIPLE QUANTUM NMR [J].