DIFFERENTIAL-EFFECTS OF INTERFERON-GAMMA AND INTERFERON-ALPHA ON INVITRO MODEL OF ANGIOGENESIS

被引：79

作者：

MAHESHWARI, RK ^{[1
]}

SRIKANTAN, V ^{[1
]}

BHARTIYA, D ^{[1
]}

KLEINMAN, HK ^{[1
]}

GRANT, DS ^{[1
]}

机构：

[1] NIDR,DEV BIOL & ANOMALIES LAB,BETHESDA,MD 20892

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1991年 / 146卷 / 01期

关键词：

D O I：

10.1002/jcp.1041460121

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (alpha) and gamma (gamma) on the capillary-like network formation in an vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-alpha enhanced the tube formation in a dose-dependent manner, whereas IFN-gamma significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-alpha and gamma was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development.

引用

页码：164 / 169

页数：6

共 21 条

[1] INHIBITION OF CELL MOTILITY BY INTERFERON [J].