DETECTION OF PEPTIDYL-3,4-DIHYDROXYPHENYLALANINE BY AMINO-ACID-ANALYSIS AND MICROSEQUENCING TECHNIQUES

被引：28

作者：

WAITE, JH

机构：

[1] Marine Biology/Biochemistry Program, College of Marine Studies, University of Delaware, Lewes

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90560-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

3,4-Dihydroxyphenylalanine (DOPA) is an amino acid that occurs naturally in the primary sequence of many proteins and peptides. Detection of peptidyl-DOPA, however, can be elusive. This is due (i) to its coelution with leucine on most of the ion exchangers used in amino acid analysis and (ii) to the coelution of phenylthiohydantoin (PTH)-DOPA with PTH-alanine during routine C18 reversed-phase HPLC following automated Edman degradation. By application of appropriately timed temperature and/or gradient modifications during chromatography, DOPA and its PTH derivative can be adequately resolved for detection by both amino acid analysis and gas-phase sequencing. © 1991.

引用

页码：429 / 433

页数：5

共 23 条

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