PROTRH-GENE EXPRESSION BY FETAL PANCREATIC-ISLETS IN CULTURE

被引:11
作者
ARATANSPIRE, S
SCHARFMANN, R
LECHAN, RM
TASHJIAN, AH
机构
[1] HARVARD UNIV,SCH MED,DEPT BIOL CHEM & MOLEC PHARMACOL,BOSTON,MA 02115
[2] HARVARD UNIV,SCH PUBL HLTH,TOXICOL LAB,BOSTON,MA 02115
[3] NEW ENGLAND MED CTR HOSP,DEPT MED,BOSTON,MA 02111
关键词
D O I
10.1016/0006-291X(90)91121-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hypothalamic tripeptide, thyrotropin-releasing hormone (TRH), has been detected in neonatal pancreatic tissue and localized by immunocytochemistry in the islets of Langerhans. To determine whether the TRH gene is expressed in islets, we have extracted RNA from cultured rat islets and probed for proTRH mRNA using a [32P]-labeled antisense RNA. Islet proTRH mRNA comigrated with the 1.6 kilobase proTRH mRNA present in the rat hypothalamus. Normalized to total RNA, islets cultured for 7 days contained at least 10 times more proTRH mRNA than day 1 whole pancreas. We conclude that pancreatic TRH is synthesized in situ in the islets of Langerhans. This is the first attempt to characterize and quantify proTRH mRNA using neoformed foetal islets. We propose that quantitative analysis of proTRH mRNA concentrations in this culture system will enable study of the direct regulation of TRH biosynthesis in the pancreas. © 1990.
引用
收藏
页码:952 / 958
页数:7
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