GLYCINE-EXTENDED PROCESSING INTERMEDIATE OF PROVIP - A NEW FORM OF VIP IN THE RAT

被引：10

作者：

FAHRENKRUG, J

机构：

[1] Department of Clinical Chemistry, Bispebjerg Hospital, University of Copenhagen

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91795-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthesis of many peptides including vasoactive intestinal polypeptide (VIP) requires enzymatic α-carboxyamidation via a glycine-extended intermediate form. In an effort to identify and quantify glycine-extended VIP in rat tissue extracts a radio-immunoassay specific for this peptide was developed. The concentrations of glycine-extended VIP ranged from 1.3 pmol/g in the brain to 83.9 pmol/g in the small intestine. The identity of the peptide was substantiated by cation-exchange HPLC. The ratio of glycine-extended VIP to amidated VIP varied considerably being highest (63%) in the small intestine. The natural occurrence of glycine-extended VIP in connection with our recent demonstration of its biological activity suggest a physiological role for this biosynthetic intermediate VIP form. © 1991.

引用

页码：173 / 177

页数：5

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