A NONRADIOACTIVE RECEPTOR ASSAY FOR SNAKE-VENOM POSTSYNAPTIC NEUROTOXINS

被引：16

作者：

STILES, BG

机构：

[1] Department of Toxinology, Pathophysiology Division, U.S. Army Medical Research Institute of Infectious Diseases, Frederick

来源：

TOXICON | 1991年 / 29卷 / 4-5期

关键词：

D O I：

10.1016/0041-0101(91)90024-L

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A non-radioactive assay was developed for detecting the binding of postsynaptic neurotoxins to acetylcholine receptor (AchR) from Torpedo californica. Enzyme linked immunosorbent assay (ELISA) wells coated with long or short chain neurotoxins specifically bound to purified AchR while crotamine or two different cardiotoxins did not. Bound receptor was detected by antibody against AchR. Specificity was determined by dose-response experiments and competition studies using carbamylcholine chloride, acetylcholine chloride, or Naja naja atra cobrotoxin mixed with receptor.

引用

页码：503 / 510

页数：8

共 28 条

[1] POSTSYNAPTIC EFFECTS OF CROTOXIN AND OF ITS ISOLATED SUBUNITS [J].

BON, C ;

CHANGEUX, JP ;

JENG, TW ;

FRAENKELCONRAT, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 99 (03) :471-481

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] ACETYLCHOLINE RECEPTORS IN NORMAL AND DENERVATED RAT DIAPHRAGM MUSCLE .2. COMPARISON OF JUNCTIONAL AND EXTRAJUNCTIONAL RECEPTORS [J].

BROCKES, JP ;

HALL, ZW .

BIOCHEMISTRY, 1975, 14 (10) :2100-2106

[4] USE OF A SNAKE VENON TOXIN TO CHARACTERIZE CHOLINERGIC RECEPTOR PROTEIN [J].

CHANGEUX, JP ;

KASAI, M ;

LEE, CY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 67 (03) :1241-+

[5] INHIBITION OF ALPHA-BUNGAROTOXIN BINDING TO ACETYLCHOLINE-RECEPTORS BY ANTISERA FROM ANIMALS WITH EXPERIMENTAL AUTOIMMUNE MYASTHENIA-GRAVIS [J].

CLAUDIO, T ;

RAFTERY, MA .

JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1980, 14 (03) :267-279

[6] COMPARISON OF THE SUBUNITS OF TORPEDO-CALIFORNICA ACETYLCHOLINE-RECEPTOR BY PEPTIDE MAPPING [J].

FROEHNER, SC ;

RAFTO, S .

BIOCHEMISTRY, 1979, 18 (02) :301-307

[7] THE APPLICATION OF LIGAND OVERLAY OF PROTEIN BLOTS TO THE STUDY OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR [J].

GERSHONI, JM .

ELECTROPHORESIS, 1987, 8 (09) :428-431

[8] BINDING OF ALPHA-BUNGAROTOXIN TO ISOLATED ALPHA-SUBUNIT OF THE ACETYLCHOLINE-RECEPTOR OF TORPEDO-CALIFORNICA - QUANTITATIVE-ANALYSIS WITH PROTEIN BLOTS [J].

GERSHONI, JM ;

HAWROT, E ;

LENTZ, TL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (16) :4973-4977

[9]

HAGGERTY JG, 1981, J BIOL CHEM, V256, P8294

[10] CROTOXIN EFFECTS ON TORPEDO-CALIFORNICA CHOLINERGIC EXCITABLE VESICLES AND ROLE OF ITS PHOSPHOLIPASE-A ACTIVITY [J].

HANLEY, MR .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 82 (01) :392-401

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