GENISTEIN, A TYROSINE KINASE INHIBITOR, BLOCKS THE CELL-CYCLE PROGRESSION BUT NOT CA2+ INFLUX INDUCED BY BAY K8644 IN FRTL-5 CELLS

被引:24
作者
TAKANO, T [1 ]
TAKADA, K [1 ]
TADA, H [1 ]
NISHIYAMA, S [1 ]
AMINO, N [1 ]
机构
[1] OSAKA UNIV,SCH MED,DEPT LAB MED,1-1-50 FUKUSHIMA,FUKUSHIMA KU,OSAKA 553,JAPAN
关键词
D O I
10.1006/bbrc.1993.1120
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
IGF-I, when added to TSH-primed FRTL-5 cells, induces a long lasting Ca2+ influx and then DNA synthesis. Ca2+ channel agonist, BAYK 8644, is capable to mimic these effects on cell proliferation. We studied the effect of genistein, a specific tyrosine kinase inhibitor, on BAY 1(8644- or IGF-I- induced cell cycle progression in FRTL-5 cells. Genitein inhibited DNA synthesis induced by BAY K8644 and by IGF-I. In contrast, Ca2+ influx stimulated by BAY K8644 was not inhibited. These data demonstrate that the signal transduction pathway induced by BAY K8644 or IGF-I may possibly involve genistein-sensitive process at the downstream step of Ca2+ entry. © 1993 Academic Press, Inc.
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页码:801 / 807
页数:7
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