AN ERCC5 GENE WITH HOMOLOGY TO YEAST RAD2 IS INVOLVED IN GROUP-G XERODERMA-PIGMENTOSUM

被引：33

作者：

SHIOMI, T ^{[1
]}

HARADA, YN ^{[1
]}

SAITO, T ^{[1
]}

SHIOMI, N ^{[1
]}

OKUNO, Y ^{[1
]}

YAMAIZUMI, M ^{[1
]}

机构：

[1] KUMAMOTO UNIV,SCH MED,INST MOLEC EMBRYOL & GENET,DEPT CELL GENET,KUMAMOTO 862,JAPAN

来源：

MUTATION RESEARCH | 1994年 / 314卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

REPAIR GENE; ERCC5; RAD2; CDNA CLONING; COMPLEMENTATION; XERODERMA PIGMENTOSUM GROUP G;

D O I：

10.1016/0921-8777(94)90080-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have isolated a human excision repair gene ERCC5 which complements the defect of the mouse UV-sensitive mutant XL216 (rodent complementation group 5). Here we report cDNA cloning of human and mouse ERCC5 genes using an exon containing an ERCC5 fragment as a probe. The ERCC5 cDNA encodes a predicted 133-kDa nuclear protein that shares some homology with the product of the yeast DNA repair gene RAD2. Transfection with mouse ERCC5 cDNA restored normal levels of UV resistance to XL216 cells. Microinjection of ERCC5 cDNA specifically restored the defect of xeroderma pigmentosum group G cells (XP-G) as measured by unscheduled DNA synthesis, and XP-G cells stably transformed with ERCCS cDNA showed nearly normal UV resistance.

引用

页码：167 / 175

页数：9

共 30 条

[1] STUDIES ON A NEW CASE OF XERODERMA PIGMENTOSUM (XP3BR) FROM COMPLEMENTATION GROUP-G WITH CELLULAR-SENSITIVITY TO IONIZING-RADIATION [J].

ARLETT, CF ;

HARCOURT, SA ;

LEHMANN, AR ;

STEVENS, S ;

FERGUSONSMITH, MA ;

MORLEY, WN .

CARCINOGENESIS, 1980, 1 (09) :745-751

[2] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[3] ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].

CHIRGWIN, JM ;

PRZYBYLA, AE ;

MACDONALD, RJ ;

RUTTER, WJ .

BIOCHEMISTRY, 1979, 18 (24) :5294-5299

[4]

Cleaver JE, 1989, METABOLIC BASIS INHE, V6th, P2949

[5] CORRECTION OF XERODERMA-PIGMENTOSUM COMPLEMENTATION GROUP-D MUTANT-CELL PHENOTYPES BY CHROMOSOME AND GENE-TRANSFER - INVOLVEMENT OF THE HUMAN ERCC2 DNA-REPAIR GENE [J].

FLEJTER, WL ;

MCDANIEL, LD ;

JOHNS, D ;

FRIEDBERG, EC ;

SCHULTZ, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :261-265

[6] DEOXYRIBONUCLEIC-ACID REPAIR IN THE YEAST SACCHAROMYCES-CEREVISIAE [J].

FRIEDBERG, EC .

MICROBIOLOGICAL REVIEWS, 1988, 52 (01) :70-102

[7] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[8] REPAIR OF DNA-CONTAINING PYRIMIDINE DIMERS [J].

GROSSMAN, L ;

CARON, PR ;

MAZUR, SJ ;

OH, EY .

FASEB JOURNAL, 1988, 2 (11) :2696-2701

[9]

HENIKOFF S, 1987, METHOD ENZYMOL, V155, P156

[10] NUCLEOTIDE-SEQUENCE, TRANSCRIPT MAPPING, AND REGULATION OF THE RAD2-GENE OF SACCHAROMYCES-CEREVISIAE [J].

MADURA, K ;

PRAKASH, S .

JOURNAL OF BACTERIOLOGY, 1986, 166 (03) :914-923

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