CRYSTAL-STRUCTURE OF INTERLEUKIN-8 - SYMBIOSIS OF NMR AND CRYSTALLOGRAPHY

被引：355

作者：

BALDWIN, ET

WEBER, IT

STCHARLES, R

XUAN, JC

APPELLA, E

YAMADA, M

MATSUSHIMA, K

EDWARDS, BFP

CLORE, GM

GRONENBORN, AM

WLODAWER, A

机构：

[1] NCI, CELL BIOL LAB, BETHESDA, MD 20892 USA

[2] DAINIPPON PHARMACEUT CO LTD, RES LAB, SUITA, OSAKA 564, JAPAN

[3] NCI, BIOL RESPONSE MODIFIERS PROGRAM, MOLEC IMMUNOREGULAT LAB, FREDERICK, MD 21702 USA

[4] WAYNE STATE UNIV, DEPT PHYSIOL, DETROIT, MI 48201 USA

[5] WAYNE STATE UNIV, SCH MED, DEPT BIOCHEM, DETROIT, MI 48201 USA

[6] NIDDKD, CHEM PHYS LAB, BETHESDA, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 02期

关键词：

CHEMOTAXIS; MOLECULAR REPLACEMENT; RECEPTOR BINDING; GROWTH FACTORS;

D O I：

10.1073/pnas.88.2.502

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 angstrom x-ray data to an R factor of 0.187 (0.217 at 1.6 angstrom). A comparison indicates some potential differences between the structure in solution and in the crystalline state. Our analysis also predicts that residues 4 through 9 on the amino terminus and the beta-bend, which includes His-33, may be important for receptor binding.

引用

页码：502 / 506

页数：5

共 36 条

[1] CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDY OF INTERLEUKIN-8 [J].