STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF RECOMBINANT-HUMAN-ERYTHROPOIETIN ANALOGS

被引：5

作者：

BITTORF, T ^{[1
]}

JASTER, R ^{[1
]}

BROCK, J ^{[1
]}

机构：

[1] UNIV ROSTOCK,FAK MED,INST MED BIOCHEM,D-18057 ROSTOCK,GERMANY

来源：

FEBS LETTERS | 1993年 / 336卷 / 01期

关键词：

ERYTHROPOIETIN; MUTAGENESIS; TRANSIENT EXPRESSION; STRUCTURE-FUNCTION RELATIONSHIP;

D O I：

10.1016/0014-5793(93)81626-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deletion mutants of a synthetic human erythropoietin-cDNA were transiently expressed in COS-7 cells and products analyzed using Western blots and a cell proliferation assay. Only two mutants with deletions affecting the N-terminal portion and the amino acid sequence 115-121 displayed biological activity. The exchange of hydrophilic, charged amino acids by alanine in two potential a-helical regions, the internal amino acid sequence 102-106 and the C-terminal sequence 154-159, causes a 2-11-fold loss of activity. The results suggest that both regions are involved in either maintaining the active structure of the hormone or interacting with the receptor.

引用

页码：133 / 136

页数：4

共 24 条

[1] HEMATOPOIETIC RECEPTORS AND HELICAL CYTOKINES
BAZAN, JF
[J]. IMMUNOLOGY TODAY, 1990, 11 (10): : 350 - 354
[2] BOISSEL JP, 1990, PROG CLIN BIOL RES, V352, P227
[3] BOISSEL JP, 1993, J BIOL CHEM, V268, P15983
[4] STRUCTURAL ROLE OF AMINO-ACIDS 99-110 IN RECOMBINANT-HUMAN-ERYTHROPOIETIN
CHERN, Y
CHUNG, T
SYTKOWSKI, AJ
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 202 (02): : 225 - 229
[5] ELECTROPORATION FOR THE EFFICIENT TRANSFECTION OF MAMMALIAN-CELLS WITH DNA
CHU, G
HAYAKAWA, H
BERG, P
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (03) : 1311 - 1326
[6] DANDREA AD, 1990, BLOOD, V75, P874
[7] DUBE S, 1988, J BIOL CHEM, V263, P17516
[8] FIBI MR, 1991, BLOOD, V77, P1203
[9] GOLDWASSER E, 1984, BLOOD CELLS, V10, P147
[10] STRUCTURE-FUNCTION-RELATIONSHIPS OF THE HEMATOPOIETIC GROWTH-FACTORS
KAUSHANSKY, K
[J]. PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1992, 12 (01): : 1 - 9

← 1 2 3 →