DNA AND RNA-POLYMERASE DNA COMPLEX IMAGED BY SCANNING FORCE MICROSCOPY - INFLUENCE OF MOLECULAR-SCALE FRICTION

被引：79

作者：

ZENHAUSERN, F

ADRIAN, M

TENHEGGELERBORDIER, B

ENG, LM

DESCOUTS, P

机构：

[1] UNIV LAUSANNE,INST BIOL ANIM,DORIGNY,SWITZERLAND

[2] UNIV LAUSANNE,ANAL ULTRASTRUCT LAB,DORIGNY,SWITZERLAND

来源：

SCANNING | 1992年 / 14卷 / 04期

关键词：

D O I：

10.1002/sca.4950140405

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Reproducible images of DNA and the interaction of RNA polymerase with supercoiled DNA on mica have been obtained by scanning force microscopy (SFM) in air. This technique allowed visualization of the assembly of distinct nucleoprotein complexes at specific sites which initiate DNA replication. Transmission electron microscopic controls were performed on the carbon-coated replica of the same specimens previously scanned by scanning force microscope. Image contrast formation has been investigated and lateral friction forces have been qualitatively characterized with SFM on DNA-covered samples.

引用

页码：212 / 217

页数：6

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