ASSAY OF THE CONCENTRATION AND C-13 ENRICHMENT OF ACETATE AND ACETYL-COA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：12

作者：

DAVID, F

BEYLOT, M

REIDER, MW

ANDERSON, VE

BRUNENGRABER, H

机构：

[1] CASE WESTERN RESERVE UNIV, DEPT NUTR, CLEVELAND, OH 44106 USA

[2] CASE WESTERN RESERVE UNIV, DEPT BIOCHEM, CLEVELAND, OH 44106 USA

[3] INSERM, U197, F-69008 LYON, FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 218卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1153

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present two techniques for determining the concentration and C-13 enrichment of acetate in biological fluids. After the sample has been spiked with an internal standard of [2,2,2,H-3(2), 1-C-13]acetate, acetate is first enzymatically converted to acetyl-coenzyme A, which is chemically converted to acetylglycine. The latter is analyzed by gas chromatography-mass spectrometry, either as a methyl ester by positive chemical ionization or as a pentafluorobenzyl ester by negative chemical ionization. The mole percentage enrichment of tissue acetyl-CoA can also be assayed after conversion to acetylglycine pentafluorobenzyl ester. (C) 1994 Academic Press, Inc.

引用

页码：143 / 148

页数：6

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