HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF CIS-TRANS ISOMERS OF PROLINE-CONTAINING PEPTIDES .2. FRACTIONATION IN DIFFERENT CYCLODEXTRIN SYSTEMS

被引：22

作者：

FRIEBE, S ^{[1
]}

HARTRODT, B ^{[1
]}

NEUBERT, K ^{[1
]}

KRAUSS, GJ ^{[1
]}

机构：

[1] MARTIN LUTHER UNIV HALLE WITTENBERG,DEPT BIOCHEM BIOTECHNOL,D-06099 HALLE,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 661卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)85172-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-Cyclodextrin-bonded silica is demonstrated to be a suitable stationary phase for high-performance liquid chromatography of conformational isomers of proline-containing peptides. In contrast to reversed-phase chromatography, the principle of inclusion complexation shows significant selectivities in conformer resolution based on a variety of interactions. New results of inclusion HPLC of biologically active oligopeptides related to beta-casomorphin on stationary phases containing bonded cyclodextrins of different internal diameters indicate a steric discrimination process during the conformer separation. beta-Cyclodextrin used as a mobile phase additive in reversed-phase systems is shown to offer the opportunity to investigate conformational changes using commercially available reversed-phase columns.

引用

页码：7 / 12

页数：6

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