PHOTOCHEMICAL LABELING OF MEMBRANE-ASSOCIATED AND CHANNEL-FORMING DOMAINS OF PROTEINS DIRECTED BY ENERGY-TRANSFER

被引：19

作者：

PENG, L ^{[1
]}

ALCARAZ, ML ^{[1
]}

KLOTZ, P ^{[1
]}

KOTZYBAHIBERT, F ^{[1
]}

GOELDNER, M ^{[1
]}

机构：

[1] UNIV LOUIS PASTEUR STRASBOURG 1, FAC PHARM, CHIM BIOORGAN LAB, CNRS, URA 1386, F-67401 ILLKIRCH GRAFFENSTADEN, FRANCE

来源：

FEBS LETTERS | 1994年 / 346卷 / 01期

关键词：

ENERGY TRANSFER; PHOTOACTIVATABLE PHOSPHOLIPID; PHOTOACTIVATABLE PERMEANT; HYDROPHOBIC LABELING; PHOTOLABELING;

D O I：

10.1016/0014-5793(94)00392-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Singlet-singlet energy transfer reactions from excited tryptophan residues to photoactivatable probes possessing a suitable chromophore, generate reactive species in the vicinity of the protein, leading to its covalent labeling. This delayed labeling process can be used to map the membrane-surrounded regions of proteins with improved efficiency when it is applied with appropriate photoactivatable phospholipids. The same principle could also be applied to the labeling of channel-forming transmembrane domains of ion channels, provided that suitable photoactivatable permeant ions were available. Both applications will be discussed with regard to their potential and feasibility.

引用

页码：127 / 131

页数：5

共 22 条

[1]

[Anonymous], 1992, ADV PHOTOCHEM, DOI DOI 10.1002/9780470133484.CH2

[2] LASER FLASH-PHOTOLYSIS STUDIES ON 4-OXOCYCLOHEXA-2,5-DIENYLIDENES [J].