BIRTH OF LAMBS AFTER INVITRO MATURATION, FERTILIZATION, AND COCULTURE WITH OVIDUCTAL CELLS

被引:34
作者
CZLONKOWSKA, M [1 ]
EYSYMONT, U [1 ]
GUSZKIEWICZ, A [1 ]
KOSSAKOWSKI, M [1 ]
DZIAK, J [1 ]
机构
[1] POLISH ACAD SCI,INST GENET & ANIM BREEDING,EMBRYO BIOTECHNOL LAB,PL-05551 MROKOW,POLAND
关键词
SHEEP OOCYTE; OVIDUCTAL EPITHELIAL CELLS;
D O I
10.1002/mrd.1080300105
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Control ovine oocytes matured and fertilized in vitro were transferred to intermediate recipient ewes. After 5 days, 59% of eggs were recovered. Thirty-one (38%) reached morula/blastocyst stage. Twenty-one embryos at the morula or blastocyst stage were transferred to six recipient ewes, resulting in five pregnancies, of which four were maintained. Nine lambs were born (43%). In the experiment, 72 oocytes matured and fertilized in vitro were cocultured for 5 days with sheep oviductal epithelial cells. Thirty-one eggs (43%) developed to the noncompacted morula stage. Transfer of 26 embryos to 11 recipient ewes resulted in two pregnancies (18%). Two male lambs were born. The result indicates that the coculture of in vitro matured and fertilized ovine eggs with sheep oviductal epithelial cells throughout the preimplantation period is compatible with further development to term.
引用
收藏
页码:34 / 38
页数:5
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