SEPARATION AND DETECTION OF PHOSPHOLIPID HYDROPEROXIDES IN THE LOW NANOMOLAR RANGE BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IRONTHIOCYANATE ASSAY

被引：27

作者：

MULLERTZ, A

SCHMEDES, A

HOLMER, G

机构：

[1] Department of Biochemistry and Nutrition, Technical University of Denmark, Lyngby, DK-2800

来源：

LIPIDS | 1990年 / 25卷 / 07期

关键词：

D O I：

10.1007/BF02537987

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the detection of phospholipid hydroperoxides in the low nanomolar range has been developed by using a high performance liquid chromatography system combined with a post-column reaction. The detection is based on the oxidation of Fe(II) to Fe(III) by hydroperoxides and the subsequent formation of an ironthiocyanate complex, which can be determined spectrophotometrically at 505 nm. By this method it is possible to separate and quantitate phosphatidylethanolamine hydroperoxide and phosphatidylcholine hydroperoxide formed in erythrocyte ghost membranes during photo-oxidation. © 1990 American Oil Chemists' Society.

引用

页码：415 / 418

页数：4

共 20 条

[1] FORMATION, REACTIONS AND TOXICITY OF ALDEHYDES PRODUCED IN THE COURSE OF LIPID-PEROXIDATION IN CELLULAR MEMBRANES [J].