ONLINE FLUORESCENCE LIFETIME DETECTION FOR CHROMATOGRAPHIC PEAK RESOLUTION

被引：18

作者：

COBB, WT ^{[1
]}

MCGOWN, LB ^{[1
]}

机构：

[1] DUKE UNIV,DEPT CHEM,PM GROSS CHEM LAB,DURHAM,NC 27706

来源：

ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 02期

关键词：

D O I：

10.1021/ac00201a019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Frequency-domain fluorescence lifetime measurements, made on-the-fly during high-performance liquid chromatography, were used to resolve overlapping chromatographic peaks of polycyclic aromatic hydrocarbons. Two-component heterogeneity analysis of the multifrequency lifetime data provided fractional intensity contributions for the individual components, which were multiplied by the total fluorescence intensity to yield the peaks of each component. Excellent results were obtained when the fluorescence lifetimes of the components were fixed In the heterogeneity analysis to values obtained from the on-the-fly lifetime chromatograms. Positive errors in the peak intensities were observed when the lifetimes were allowed to float in the heterogeneity analysis; the source of the error has not yet been identified but may be due to systematic bias in the heterogeneity analysis software. © 1990, American Chemical Society. All rights reserved.

引用

页码：186 / 189

页数：4

共 8 条

[1] MULTIFREQUENCY PHASE-MODULATION FLUORESCENCE LIFETIME DETERMINATIONS ON-THE-FLY IN HPLC [J].