RESISTANCE TO HUMAN RESPIRATORY SYNCYTIAL VIRUS (RSV) INFECTION INDUCED BY IMMUNIZATION OF COTTON RATS WITH A RECOMBINANT VACCINIA VIRUS EXPRESSING THE RSV-G GLYCOPROTEIN

被引：107

作者：

ELANGO, N

PRINCE, GA

MURPHY, BR

VENKATESAN, S

CHANOCK, RM

MOSS, B

机构：

[1] NIAID, VIRAL DIS LAB, BETHESDA, MD 20892 USA

[2] NIAID, INFECT DIS LAB, BETHESDA, MD 20892 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1986年 / 83卷 / 06期

关键词：

D O I：

10.1073/pnas.83.6.1906

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A cDNA copy of the G glycoprotein gene of human respiratory syncytial virus (RSV) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and expressed a 93-kDa protein that migrated with the authentic RSV G glycoprotein upon polyacrylamide gel eletrophoresis. Glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of other RSV proteins. Cotton rats that were inoculated intradermally with the infectious recombinant virus produced serum antibody to the G glycoprotein that neutralized RSV in vitro. Furthermore, the vaccinated animals were resistant to lower respiratory tract infection upon intranasal inoculation with RSV and had reduced titrs of RSV in the nose.

引用

页码：1906 / 1910

页数：5

共 45 条

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