SYNTHESIS OF 2-DEOXY-ALPHA-D-ARABINO-HEXOPYRANOSYL PHOSPHATE AND 2-DEOXY-MALTOOLIGOSACCHARIDES WITH PHOSPHORYLASE

被引：31

作者：

EVERS, B ^{[1
]}

MISCHNICK, P ^{[1
]}

THIEM, J ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ORGAN CHEM,D-20146 HAMBURG,GERMANY

来源：

CARBOHYDRATE RESEARCH | 1994年 / 262卷 / 02期

关键词：

ENZYMATIC SYNTHESIS; PHOSPHORYLASE; 2-DEOXY-ALPHA-D-ARABINO-HEXOPYRANOSYL PHOSPHATE; 2-DEOXYAMYLOSE;

D O I：

10.1016/0008-6215(94)84189-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A convenient one-step synthesis of 2-deoxy-alpha-D-arabino-hexopyranosyl phosphate on a millimolar scale is described by reaction of potato phosphorylase with D-glucal at equimolar phosphate concentration. Furthermore, in the presence of catalytic amounts of phosphate, a 2-deoxy-maltooligosaccharide is obtained from maltotetraose and D-glucal. The water,insoluble oligosaccharide was isolated and characterized by H-1 and C-13 NMR spectroscopy. An average dp of 20 was thus determined.

引用

页码：335 / 341

页数：7

共 23 条

[1]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[2] PRIMER SPECIFICITY OF POTATO PHOSPHORYLASE [J].