PRIMARY SEQUENCE, TISSUE-SPECIFICITY AND MESSENGER-RNA EXPRESSION OF THE NA+,K+-ATPASE BETA-1 SUBUNIT IN THE EUROPEAN EEL (ANGUILLA-ANGUILLA)

被引：32

作者：

CUTLER, CP

SANDERS, IL

HAZON, N

CRAMB, G

机构：

[1] School of Biological and Medical Sciences, Bute Medical Buildings, University of St Andrews, St Andrews, KY16 9TS, Fife

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 1995年 / 14卷 / 05期

关键词：

ADAPTATION; ANGUILLA ANGUILLA; BETA-1; ISOFORM; CDNA; EEL; GILL; MESSENGER-RNA; NA+; K+-ATPASE; NUCLEOTIDE SEQUENCE; PCR;

D O I：

10.1007/BF00003380

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The entire amino acid coding sequence of the Na+,K+-ATPase beta 1 isoform was cloned from the gill of the European eel (Anguilla anguilla) by a PCR based method. The amino acid sequence translated from the nucleotide sequence shared 61.4 and 56.2% homology respectively with previously published Na+,K+ ATPase beta 1 isoform sequences from the clawed toad (Xenopus laevis) and the ray (Torpedo californica) an elasmobranch fish. The size of the Na+,K+-ATPase beta 1 mRNA transcript in eel tissues was demonstrated to be 2.35 Kb. Detectable levels of Na+,K+-ATPase beta 1 mRNA were found at some level in all tissues except liver and cardiac muscle. The level of branchial Na+,K+-ATPase beta 1 mRNA was observed to increase after the adaptation of fresh water eels to normal or double concentration sea water.

引用

页码：423 / 429

页数：7

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