Antihepatotoxic activity of icariin, a major constituent of Epimedium koreanum

被引:64
作者
Lee, MK [1 ]
Choi, YJ [1 ]
Sung, SH [1 ]
Shin, DI [1 ]
Kim, JW [1 ]
Kim, YC [1 ]
机构
[1] SEOUL NATL UNIV, COLL PHARM, SEOUL 151742, SOUTH KOREA
关键词
antihepatotoxic activity; CCl4; Epimedium koreanum; Berberidaceae; flavonol; icariin; primary cultured rat hepatocytes;
D O I
10.1055/s-2006-959362
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In an attempt to identify compounds with antihepatotoxic activity, carbon tetrachloride-induced cytotoxicity in primary cultured rat hepatocytes has been adopted as a screening system. Using this screening system, an antihepatotoxic compound from the aerial parts of Epimedium koreanum has been isolated. This compound, icariin, is a flavonol glycoside. Its antihepatotoxic activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase hom CCl4-intoxicated rat hepatocytes into the culture medium. Icariin significantly reduced the level of glutamic pyruvic transaminase and sorbitol dehydrogenase released resulting in a 76% protection from toxicity at concentration ranges from 1 mu M to 20 mu M. The antihepatotoxic activity of icariin was also estimated by the determination of total cytochrome P-450 content and glutathione-S-transferase activity in the CCl4-intoxicated hepatocytes.
引用
收藏
页码:523 / 526
页数:4
相关论文
共 16 条
[1]  
ASADA M, 1963, GASTROENTEROLOGY, V44, P578
[2]   HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY [J].
BERRY, MN ;
FRIEND, DS .
JOURNAL OF CELL BIOLOGY, 1969, 43 (03) :506-+
[3]  
Berry MN, 1991, LABORATORY TECHNIQUE, V21, P15
[4]  
HABIG WH, 1974, J BIOL CHEM, V249, P7130
[5]   3 FLAVONOL GLYCOSIDES FROM EPIMEDIUM-KOREANUM [J].
ITO, Y ;
HIRAYAMA, F ;
SUTO, K ;
SAGARA, K ;
YOSHIDA, T .
PHYTOCHEMISTRY, 1988, 27 (03) :911-913
[6]  
Kang S. S., 1990, KOR J PHARMACOGN, V21, P56
[7]   LIVER-PROTECTIVE DRUGS .6. ASSAY-METHOD FOR ANTIHEPATOTOXIC ACTIVITY USING CARBON-TETRACHLORIDE INDUCED CYTO-TOXICITY IN PRIMARY CULTURED-HEPATOCYTES [J].
KISO, Y ;
TOHKIN, M ;
HIKINO, H .
PLANTA MEDICA, 1983, 49 (04) :222-225
[8]   PREPARATION OF COLLAGEN SUBSTRATES FOR CELL ATTACHMENT - EFFECT OF COLLAGEN CONCENTRATION AND PHOSPHATE BUFFER [J].
KLEINMAN, HK ;
MCGOODWIN, EB ;
RENNARD, SI ;
MARTIN, GR .
ANALYTICAL BIOCHEMISTRY, 1979, 94 (02) :308-312
[9]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[10]   NEW FLAVONOL GLYCOSIDE FROM THE LEAVES OF EPIMEDIUM-SEMPERVIRENS [J].
MIZUNO, M ;
IINUMA, M ;
TANAKA, T ;
SAKAKIBARA, N .
JOURNAL OF NATURAL PRODUCTS, 1990, 53 (03) :744-746