NEW EFFICIENT SULFURIZING REAGENTS FOR THE PREPARATION OF OLIGODEOXYRIBONUCLEOTIDE PHOSPHOROTHIOATE ANALOGS

被引：33

作者：

EFIMOV, VA ^{[1
]}

KALINKINA, AL ^{[1
]}

CHAKHMAKHCHEVA, OG ^{[1
]}

HILL, TS ^{[1
]}

JAYARAMAN, K ^{[1
]}

机构：

[1] TRIPLEX PHARMACEUT CORP,THE WOODLANDS,TX 77380

来源：

NUCLEIC ACIDS RESEARCH | 1995年 / 23卷 / 20期

关键词：

D O I：

10.1093/nar/23.20.4029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A set of new sulfurizing agents representing disulfides of arylsulfonic acids has been developed for the automated synthesis of phosphorothioate oligonucleotide analogues via the phosphoramidite method. These reagents, such as bis(benzenesulfonyl)disulfide, bis(p-toluenesulfonyl)disulfide, bis(p-methoxybenzensulfonyl)disulfide, and bis (p-chlorobenzenesulfonyl) disulfide, are easily prepared crystalline solid compounds. They are relatively inexpensive, easy to handle, and efficiently Convert internucleotide cyanoethyl phosphite to the phosphorothioate triester within 1-2 min. The efficiency of phosphorothioate oligonucleotide synthesis with the use of these reagents is comparable to that of phosphodiester oligonucleotides.

引用

页码：4029 / 4033

页数：5

共 17 条

[1]

ATKINSON T, 1984, OLIGONUCLEOTIDE SYNT, P35

[2] DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS [J].