GENOMIC LOCALIZATION OF HOG-CHOLERA VIRUS GLYCOPROTEINS

被引：54

作者：

STARK, R ^{[1
]}

RUMENAPF, T ^{[1
]}

MEYERS, G ^{[1
]}

THIEL, HJ ^{[1
]}

机构：

[1] FED RES CTR VIRUS DIS ANIM,POB 1149,W-7400 TUBINGEN,GERMANY

来源：

VIROLOGY | 1990年 / 174卷 / 01期

关键词：

D O I：

10.1016/0042-6822(90)90076-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A polyspecific antiserum has been used to identify four different glycoproteins in hog cholera virus (HCV)-infected cells termed gp55, gp48, gp44, and gp33 (Rümenapf et al, 1989, Virology 171, 18-27). Fusion proteins containing parts of the putative HCV-encoded glycoproteins were expressed in bacteria and served for the preparation of specific antibodies. These were used in radioimmunoprecipitation assays which revealed that gp48 and gp44 most likely share a common protein backbone. The order of the glycoproteins on the HCV genome was determined as follows: NH2-gp44/gp48-gp33-gp55-COON. © 1990.

引用

页码：286 / 289

页数：4

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