PRODUCTION OF 5-METHYLURIDINE BY IMMOBILIZED THERMOSTABLE PURINE NUCLEOSIDE PHOSPHORYLASE AND PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE FROM BACILLUS-STEAROTHERMOPHILUS JTS-859

被引：35

作者：

HORI, N

WATANABE, M

SUNAGAWA, K

UEHARA, K

MIKAMI, Y

机构：

[1] Tobacco Science Research Laboratory, Japan Tobacco Inc., Yokohama

来源：

JOURNAL OF BIOTECHNOLOGY | 1991年 / 17卷 / 02期

关键词：

BACILLUS-STEAROTHERMOPHILUS; 5-METHYLURIDINE; THERMOSTABLE PURINE NUCLEOSIDE PHOSPHORYLASE; THERMOSTABLE PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE;

D O I：

10.1016/0168-1656(91)90003-E

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60-degrees-C for 17 d without any bacterial contamination under non-aseptical conditions. Half-lives of the activity of the immobilized enzymes were 47 d and 4.5 d at 60-degrees-C and 70-degrees-C, respectively, although half-life of the crude enzyme was only 14 h at 70-degrees-C.

引用

页码：121 / 131

页数：11

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