RETAINED ACTIVITIES OF SOME MEMBRANE-PROTEINS IN STABLE LIPID BILAYERS ON A SOLID SUPPORT

被引：48

作者：

PUU, G

GUSTAFSON, I

ARTURSSON, E

OHLSSON, PA

机构：

[1] National Defence Research Establishment, Department of NBC Defence

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 05期

关键词：

ACETYLCHOLINESTERASE; ATOMIC FORCE MICROSCOPY; BACTERIORHODOPSIN; BIOSENSOR; LIPOSOME; NICOTINIC ACETYLCHOLINE RECEPTOR;

D O I：

10.1016/0956-5663(95)96892-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Highly stable lipid bilayers, composed of biologically relevant lipids such as phosphatidylcholine, phosphatidylethanolamine and cholesterol, were formed on platinum surfaces. Bacteriorhodopsin isolated from purple membrane (PM) from Halobacterium halobium, cytochrome oxidase from bovine heart, acetylcholinesterase from bovine brain and the nicotinic acetylcholine receptor from Torpedo electric organ were also incorporated into these reconstituted membranes. The proteins retained their biological activities. Some of them were active several weeks after the reconstitution and after several cycles of assay, washing and storage.

引用

页码：463 / 476

页数：14

共 29 条

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