RESTRICTED DIFFUSION OF INTEGRAL MEMBRANE-PROTEINS AND POLYPHOSPHOINOSITIDES LEADS TO THEIR DEPLETION IN MICROVESICLES RELEASED FROM HUMAN ERYTHROCYTES

被引：64

作者：

HAGELBERG, C ^{[1
]}

ALLAN, D ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,UNIV COLL & MIDDLESEX SCH MED,DEPT PHYSIOL,ROCKEFELLER BLDG,LONDON WC1E 6JJ,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1990年 / 271卷 / 03期

关键词：

D O I：

10.1042/bj2710831

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein and phospholipid composition of microvesicles released from normal human erythrocytes after ATP depletion, on aging or by treatment with merocyanine 540, dimyristoyl phosphatidylcholine or Ca2+/ionophore A23187 has been compared with the composition of the original cell membrane. It has been shown that these microvesicles are depleted of band 3, glycophorin and phosphatidylinositol 4,5-bisphosphate relative to phospholipid by 40% or more. These data are interpreted to mean that less than half of these membrane components are free to diffuse laterally in the lipid bilayer. Acetylcholinesterase was found to be enriched 2-3-fold in microvesicles, possibly because the removal of non-diffusing proteins from the vesiculating region of the lipid bilayer allows more space for freely diffusing proteins like acetylcholinesterase to enter the microvesicle membrane.

引用

页码：831 / 834

页数：4

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