REDUCTIVE ALKYLATION OF LIPASE

被引：20

作者：

AMPON, K ^{[1
]}

SALLEH, AB ^{[1
]}

SALAM, F ^{[1
]}

YUNUS, WMZW ^{[1
]}

RAZAK, CNA ^{[1
]}

BASRI, M ^{[1
]}

机构：

[1] UNIV AGR MALAYSIA,FAC SCI & ENVIRONM STUDIES,DEPT CHEM,SERDANG,MALAYSIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 07期

关键词：

REDUCTIVE ALKYLATION; LIPASE; ESTEROLYTIC; ESTERIFICATION; LIVER ALCOHOL-DEHYDROGENASE; AMINO-GROUPS; ORGANIC-SOLVENTS; PROTEINS; CATALYSIS; IMMOBILIZATION; SUBSTITUENTS; SURFACE; BEADS;

D O I：

10.1016/0141-0229(91)90097-T

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase (triacylglycerol acylhydrolase; EC: 3.1.1.3) from Candida rugosa has been chemically modified by reductive alkylation with aldehydes of various chain length and steric hindrance. Modified lipase showed reduced esterolytic activity but higher specific activity of esterification compared to native enzyme. The maximal esterification activity of the alkylated lipase depends on its degree of derivatization by the respective aldehydes. Lipase derivatized with octaldehyde and dodecylaldehyde are more thermostable than those modified with aldehydes with shorter chain length. Alkylated lipases are relatively more stable in organic solvent than native enzyme.

引用

页码：597 / 601

页数：5

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