MOLECULAR-CLONING AND EXPRESSION OF THIOBACILLUS-FERROOXIDANS CHROMOSOMAL RIBULOSE BISPHOSPHATE CARBOXYLASE GENES IN ESCHERICHIA-COLI

被引：22

作者：

KUSANO, T ^{[1
]}

SUGAWARA, K ^{[1
]}

INOUE, C ^{[1
]}

SUZUKI, N ^{[1
]}

机构：

[1] DOWA MIN CO LTD,RES CTR,TOKYO,JAPAN

来源：

CURRENT MICROBIOLOGY | 1991年 / 22卷 / 01期

关键词：

D O I：

10.1007/BF02106210

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes coding for d-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium, Thiobacillus ferrooxidans, were cloned into an Escherichia coli plasmid, pUC18. The recombinant plasmid, termed pTR11, contained a 4.0-kb PstI fragment including the entire coding regions for both large and small subunits of RuBPCase. Escherichia coli carrying pTR11 did not show any CO2-fixing activity. However, a derivative plasmid with an appropriate deletion, which was placed under the control of a tac promoter, conferred ribulose bisphosphate-dependent CO2-fixing activity on the host cell. Analysis of gel-filtration chromatography of the RuBPCase synthesized in E. coli revealed that it had a hexadecameric form like the native enzyme of T. ferrooxidans. © 1991 Springer-Verlag New York Inc.

引用

页码：35 / 41

页数：7

共 32 条

[1] MUTATIONS ALTERING THE CATALYTIC ACTIVITY OF A PLANT-TYPE RIBULOSE BISPHOSPHATE CARBOXYLASE-OXYGENASE IN ALCALIGENES-EUTROPHUS [J].