BIOTRANSFORMATION OF 4-NITROPHENOL BY A FERMENTER GROWN CELL-SUSPENSION CULTURE OF SOYBEAN (GLYCINE-MAX) - ISOLATION AND IDENTIFICATION OF CONJUGATES

被引：11

作者：

KNOPS, M ^{[1
]}

SCHUPHAN, I ^{[1
]}

SCHMIDT, B ^{[1
]}

机构：

[1] TECH UNIV AACHEN, INST BIOL 5, D-52056 AACHEN, GERMANY

来源：

PLANT SCIENCE | 1995年 / 109卷 / 02期

关键词：

GLYCINE MAX; CELL SUSPENSION CULTURE; 4-NITROPHENOL; AIRLIFT FERMENTER; NMR SPECTROSCOPY; BIOTRANSFORMATION;

D O I：

10.1016/0168-9452(95)04175-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fermenter grown cell suspension culture of soybean (Glycine max L.) metabolized 4-nitrophenol by glucosylation to 1(O-beta-D-glucopyranosyl)-4-nitrobenzene, and subsequently processed the P-glucoside by malonylation at position 6 of the glucose moiety yielding 1(6'-O-malonyl-O-beta-D-glucopyranosyl)-4-nitrobenzene. With regard to the soluble metabolites, the soybean cells cultivated in the modified airlift fermenter (3-1 working volume) did not show any significant differences to the corresponding batch cultures grown in 100-ml shake flasks. The metabolites accumulating in the cells were identified by TLC, HPLC, H-1- and C-13-NMR spectroscopy.

引用

页码：215 / 224

页数：10

共 31 条

[1] THIN-LAYER CHROMATOGRAPHY OF CARBOHYDRATES IN PRESENCE OF BISULFITE [J].