THE V-REL ONCOGENE PRODUCT IS COMPLEXED WITH CELLULAR PROTEINS INCLUDING ITS PROTOONCOGENE PRODUCT AND HEAT-SHOCK PROTEIN-70

被引:39
作者
LIM, MY
DAVIS, N
ZHANG, JY
BOSE, HR
机构
[1] UNIV TEXAS,DEPT MICROBIOL,AUSTIN,TX 78712
[2] UNIV TEXAS,CELL RES INST,AUSTIN,TX 78712
关键词
D O I
10.1016/0042-6822(90)90195-W
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The oncogene product, pp59v-rel of avian reticuloendotheliosis virus (REV-T) is complexed in the cytosol of REV-T transformed lymphoid cells with cellular proteins. Monoclonal antibodies and antisera directed against different regions of pp59v-rel coimmunoprecipitate five cellular proteins (pl24, pl 15, p75, p70, and p40) in addition to pp59v-rel. Cellular proteins with the same apparent molecular mass also copurify with pp59v-rel during sequential Sephacryl S200 and immunoaffinity chromatography. Antisera directed against the most abundant cellular protein in the complex, pp40, coimmunoprecipitate pp59v-rel and several cellular proteins with the same apparent molecular mass. The 75-kDa protein in the pp59v-rel complex is the product of c-rel proto-oncogene and is weakly phosphorylated. In MSB-1 cells this protein is not detectably phosphorylated or associated with cellular proteins. The 70-kDa protein in the pp59v-rel containing cytosolic complex is the constitutive form of avian heat shock protein 70 (HSC70). The p70 protein coimmunoprecipitates and copurifies with pp59v-rel using antisera directed against pp59v-rel and coimmunoprecipitates with antisera specific for pp40. The p70 isolated from immune complexes containing pp59v-rel shares V8 protease fragments with HSC70. © 1990.
引用
收藏
页码:149 / 160
页数:12
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